Abstract:

Objective: The purpose of this paper is to differentiate and analyze the term concepts of biuret method and Folin-Phenol method, improve the operation method of measuring serum total protein by the two methods, and to discuss the applicability of these two methods to detect serum total protein content in teaching, and to provide improved ideas for clinical detection of serum total protein. Methods: The improved biuret method and Folin-Phenol method were used to determine the total protein content in the serum of 40 healthy people, and the precision, repeatability and the sample recovery rate of the standard curve were investigated. Results: The total protein content in the serum samples detected by the two methods was basically consistent with the hospital test results. Conclusion: Both methods are suitable for the determination of serum total protein content. Due to the short detection time and simple operation, the biuret method is more suitable for experimental teaching than the Folin-Phenol method, which is worthy of further promotion.

Key words: biuret method, Folin-Phenol method, determination of serum total protein