双缩脲法与福林酚法辨析及应用评价

doi:10.12339/j.issn.1673-8578.2025.01.021

中国科技术语 ›› 2025, Vol. 27 ›› Issue (1): 142-148.doi: 10.12339/j.issn.1673-8578.2025.01.021

双缩脲法与福林酚法辨析及应用评价

章倩(), 林燕燕, 蔡彬祥^*()

漳州卫生职业学院基础医学科研中心,福建漳州 363000

收稿日期:2024-08-30 修回日期:2024-10-28 出版日期:2025-01-07 发布日期:2025-01-07
通讯作者:
^* 蔡彬祥(1992—),博士,漳州卫生职业学院讲师,研究方向为生物化学与分子生物学研究。通信方式:2267217008@qq.com。
作者简介:
章倩(1986—),漳州卫生职业学院主管护师,研究方向为海洋天然产物活性研究、生物化学与生物化学检验、综合实训管理研究,通信方式:303916828@qq.com。
基金资助:
2023年度福建省中青年教师科研项目(科技类)(JZ230086); 2023漳州市自然科学基金(ZZ2023J25)

Application Evaluation of Improved Biuret Method and Folin-Phenol Reagent Method for Determination of Serum Total Protein Content

ZHANG Qian(), LIN Yanyan, CAI Binxiang^*()

Received:2024-08-30 Revised:2024-10-28 Online:2025-01-07 Published:2025-01-07

摘要/Abstract

摘要：

文章旨在辨析双缩脲法和福林酚法的术语概念,研究改进二者测量血清总蛋白的操作方法,探讨这两种方法检测血清总蛋白含量在教学中的适用性,为临床上对血清总蛋白的检测提供改良思路。通过改进双缩脲法和福林酚法分别对40个健康体检人员的血清进行总蛋白含量测定,并考察标准曲线的精密度、重复性以及加样回收率。结果发现:两种方法检测待测血清样品中总蛋白含量与医院检测结果基本一致。可见,两种方法均适用于血清总蛋白含量的测定。由于双缩脲法检测时间短、操作简单,比福林酚法更适用于实验教学,值得进一步推广。

关键词: 双缩脲法, 福林酚法, 血清总蛋白测定

Abstract:

Objective: The purpose of this paper is to differentiate and analyze the term concepts of biuret method and Folin-Phenol method, improve the operation method of measuring serum total protein by the two methods, and to discuss the applicability of these two methods to detect serum total protein content in teaching, and to provide improved ideas for clinical detection of serum total protein. Methods: The improved biuret method and Folin-Phenol method were used to determine the total protein content in the serum of 40 healthy people, and the precision, repeatability and the sample recovery rate of the standard curve were investigated. Results: The total protein content in the serum samples detected by the two methods was basically consistent with the hospital test results. Conclusion: Both methods are suitable for the determination of serum total protein content. Due to the short detection time and simple operation, the biuret method is more suitable for experimental teaching than the Folin-Phenol method, which is worthy of further promotion.

Key words: biuret method, Folin-Phenol method, determination of serum total protein

章倩, 林燕燕, 蔡彬祥. 双缩脲法与福林酚法辨析及应用评价[J]. 中国科技术语, 2025, 27(1): 142-148.

ZHANG Qian, LIN Yanyan, CAI Binxiang. Application Evaluation of Improved Biuret Method and Folin-Phenol Reagent Method for Determination of Serum Total Protein Content[J]. China Terminology, 2025, 27(1): 142-148.

图/表 10

参考文献 6

[1]	代新华. 血清与血浆总蛋白双缩脲法测定结果的分析[J]. 心理月刊, 2019(9):114-115.
[2]	纪圆圆, 高华峰, 董玲玉, 等. ZnS量子点荧光分光光度法检测牛血清白蛋白含量的研究[J]. 北京化工大学学报(自然科学版), 2016(5):69-73. doi: 10.13543/j.bhxbzr.2016.05.012
[3]	范婵, 刘艳婷, 赵印, 等. 总蛋白双缩脲法单试剂盒与双试剂盒检测结果的一致性评估[J]. 医学信息, 2018(20):152-154.
[4]	张玲, 石彩文, 肖凯军, 等. 双缩脲法测定罗非鱼源胶原蛋白肽含量的改良及应用评价[J]. 食品科学, 2019(20):234-240. doi: 10.7506/spkx1002-6630-20181118-206
[5]	王谦. 改良双缩脲法测定血清总蛋白的方法与应用[J]. 中国厂矿医学, 2007(6):668-669.
[6]	苗会娟, 胡卫国, 徐艳梅, 等. 福林酚分光光度法测定蜂毒中总蛋白质[J]. 化学分析计量, 2022(7):5-8.

标准液浓度 (mg/mL)	17.6	35.2	52.8
吸光值	0.151	0.223	0.294
	0.15	0.222	0.295
	0.149	0.222	0.299
	0.153	0.224	0.302
	0.151	0.226	0.298
	0.15	0.225	0.297
平均值	0.001247	0.002082	0.00263
RSD(%)	0.8278	0.929315	0.884019

标准液浓度 (mg/mL)	17.6	35.2	52.8
吸光值	0.151	0.223	0.294
	0.15	0.222	0.295
	0.149	0.222	0.299
	0.153	0.224	0.302
	0.151	0.226	0.298
	0.15	0.225	0.297
平均值	0.001247	0.002082	0.00263
RSD(%)	0.8278	0.929315	0.884019

样品吸光值		平均值	RSD(%)
0.363	73.84210526	74.5	1.3944
0.366	74.63157895
0.372	76.21052632
0.362	73.57894737
0.369	75.42105263
0.361	73.31578947

样品吸光值		平均值	RSD(%)
0.363	73.84210526	74.5	1.3944
0.366	74.63157895
0.372	76.21052632
0.362	73.57894737
0.369	75.42105263
0.361	73.31578947

医院测定血清总蛋白浓度(mg/mL)	样品吸光值	实测总蛋白浓度(mg/mL)	回收率(%)	平均值	RSD(%)
64.8	0.284	71.10526	103.9551	106.6482	1.851227
	0.292	75.31579	110.1108
	0.286	72.15789	105.494
	0.288	73.21053	107.0329
	0.289	73.73684	107.8024
	0.286	72.15789	105.494

双缩脲法与福林酚法辨析及应用评价

Application Evaluation of Improved Biuret Method and Folin-Phenol Reagent Method for Determination of Serum Total Protein Content

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血清编号	医院测定血清总蛋白浓度	实测血清总蛋白浓度平均值	血清编号	医院测定血清总蛋白浓度	实测血清总蛋白浓度平均值
1	59.3	63.1	21	69.7	69.7
2	73.3	75.1	22	62.8	69.5
3	76.7	76.9	23	76.8	78.1
4	69.5	69.2	24	62.3	76.3
5	64.2	63.7	25	70.3	73.3
6	65.7	68.2	26	69.7	67.6
7	64.8	65.6	27	72.7	72.6
8	68.4	66.6	28	67	65.3
9	72	73.6	29	71	75.3
10	79.3	78.8	30	59.5	63.6
11	71.8	71.7	31	53.9	55.4
12	74.3	76.3	32	66	72.4
13	68.2	70.3	33	57.5	64.8
14	76.4	79.6	34	68.1	72.3
15	68.6	68.0	35	69.3	75.1
16	71.9	69.7	36	74.7	70.3
17	64.2	65.5	37	65	69.2
18	65.6	66.4	38	73.7	70.5
19	73.3	73.1	39	70.5	67.6
20	66	70.8	40	58.9	61.7

标准液浓度 (mg/mL)	2.2	4.4	8.8
吸光值	0.136	0.204	0.34
	0.133	0.206	0.339
	0.133	0.207	0.344
	0.134	0.205	0.346
	0.133	0.201	0.345
	0.135	0.203	0.338
平均值	0.134	0.204333	0.342
RSD(%)	0.8617	0.9651	0.9091

样品吸光值	血清总蛋白浓度(mg/mL)	平均值 (mg/mL)	RSD(%)
0.288	74.11371237	74.503902	1.520455
0.285	73.11036789
0.293	75.78595318
0.291	75.11705686
0.293	75.78595318
0.285	73.11036789

医院测定血清总蛋白浓度(mg/mL)	样品吸光值	实测血清总蛋白浓度(mg/mL)	回收率(%)	平均值(%)	RSD(%)
68.4	0.309	74.7742475	1.093191	111.1119	1.7169
	0.311	76.1120401	1.112749
	0.308	74.1053512	1.083412
	0.312	76.7809365	1.122528
	0.311	76.1120401	1.112749
	0.314	78.1187291	1.142087

血清编号	医院测定血清总蛋白浓度	实测血清总蛋白浓度平均值	血清编号	医院测定血清总蛋白浓度	实测血清总蛋白浓度平均值
1	59.3	63.2	21	69.7	72.4
2	73.3	73.1	22	62.8	63.8
3	76.7	72.1	23	76.8	71.9
4	69.5	70.0	24	62.3	67.0
5	64.2	63.2	25	70.3	69.3
6	65.7	65.2	26	69.7	67.1
7	64.8	64.9	27	72.7	70.0
8	68.4	69.9	28	67	70.4
9	72	70.3	29	71	73.0
10	79.3	78.5	30	59.5	60.2
11	71.8	75.3	31	53.9	57.5
12	74.3	76.8	32	66	64.6
13	68.2	70.1	33	57.5	60.8
14	76.4	77.6	34	68.1	69.1
15	68.6	69.6	35	69.3	72.9
16	71.9	70.1	36	74.7	79.3
17	64.2	69.1	37	65	63.6
18	65.6	71.2	38	73.7	76.9
19	73.3	75.2	39	70.5	66.8
20	66	72.8	40	58.9	64.2